merck millipore,默克密理博,MAB377X,Anti-NeuN Antibody, clone A60, Alexa Fluor488 conjugated

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merck millipore,默克密理博,MAB377X,Anti-NeuN Antibody, clone A60, Alexa Fluor488 conjugated
产品名称：Anti-NeuN Antibody, clone A60, Alexa Fluor488 conjugated
产品型号：MAB377X
Anti-NeuN Antibody, clone A60, Alexa Fluor488 conjugated is an antibody against NeuN for use in IH.

merck millipore,默克密理博,MAB377X,Anti-NeuN Antibody, clone A60, Alexa Fluor488 conjugated

产品介绍

merck millipore,默克密理博,MAB377X,Anti-NeuN Antibody, clone A60, Alexa Fluor488 conjugated

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	IHC	M	AlexaFluor®488	Monoclonal Antibody

描述
产品目录编号	MAB377X
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-NeuN Antibody, clone A60, Alexa Fluor®488 conjugated
Alternate Names	Neuron-Specific Nuclear Protein
背景信息	NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).

产品信息
格式	AlexaFluor®488
控制	Brain tissue, most neuronal cell types throughout the adult nervous system
演示	Purified immunoglobulin conjugated to Alexa Fluor® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide.

应用
应用	Anti-NeuN Antibody, clone A60, Alexa Fluor®488 conjugated is an antibody against NeuN for use in IH.
主要应用	Immunohistochemistry
应用说明	Immunohistochemistry: 1:100 on rat (paraformaldehyde fixed) and mouse (paraformaldehyde fixed, antigen retrieved) brain tissue. Optimal working dilutions must be determined by end user.

生物信息
免疫原品种	Purified cell nuclei from mouse brain.
克隆	A60
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei). MAB377X reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. The immunohistochemical staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. The few cell types not reactive with MAB377X include Purkinje, mitral and photoreceptor cells. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones. The antibody is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants.
同种型	IgG1
品种反应性	RatHumanMouse
抗体类型	Monoclonal Antibody
基因符号	A60 NeuN
纯化方法	Protein A purfied

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

储存和货运信息
存储条件	Maintain for 6 months at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.